Hair Growth<\/strong><\/h3>\n\n\n\nFullerenes in general have been pinpointed as potential beneficial ingredients in cosmetics and hair-health products. Some science supports the theory that C60 <\/sub>enhances the strength of human hair fibers. In a 2006 study published by Applied Surface Science<\/em>, researchers compared C60<\/sub>+ and Ga+ (positive gallium ions) in the enhancement of hair fibers in vitro<\/em>. While at first data for the two ions appeared to be on a similar positive spectrum, further investigation yielded that C60<\/sub>+ produced a positive enhancement factor at silicone and cationic conditioner peaks that exceeded Ga+ by two and three orders of magnitude.<\/p>\n\n\n\nSome have theorized that C60 <\/sub>may have the capacity to be an effective hair-protective agent based on its purported anti-aging properties as well as its protective capabilities against chemotherapy-induced detrimental effects (possibly including hair loss). However, peer-reviewed, placebo-controlled clinical research is very limited in this regard.<\/p>\n\n\n\nCancer<\/strong><\/h3>\n\n\n\nIn recent years, scientists have explored nanotechnology and its applications in novel cancer therapies. Buckminsterfullerene has emerged as a potential therapeutic in the field of cancer diagnosis and treatment. A 1994 study published by Chinese Physics Letters<\/em> observed the biological impact of a water-soluble C60 <\/sub>liposome on human cervix cancer cells. Results indicated a significant inhibitory affect on cervix cancer cells when irradiated with C60<\/sub>.<\/p>\n\n\n\nA 2008 study published by Biomaterials <\/em>further elucidated the molecule\u2019s possible anti-cancer mechanism. Researchers administered a preparation of C60 <\/sub>fullerene (at doses of 25, 50, and 100 mg\/kg per week for three weeks) to male Wistar rats with doxorubicin-induced toxicity as well as colorectal cancer. After results were investigated on macroscopic, microscopic, hematological, biochemical, physiological, pharmacological, and pharmacokinetic parameters, researchers concluded that C60 <\/sub>exerted a protective effect on the liver and tissue against chemotherapy-induced chronic toxicity. However, its ameliorative effects against colorectal cancer cells were not significant.<\/p>\n\n\n\nThere is some concern that C60 <\/sub>fullerene may yield considerable toxicity toward normal cells, which lends some confusion towards its anti-cancer potential. A 2009 study also published by Biomaterials<\/em> observed the ability of C60<\/sub> to suppress tumor cell growth in vitro<\/em> and in vivo<\/em>. In the initial stages of the study, researchers observed that C60<\/sub> induced apoptotic and necrotic cell death in B16 melanoma cells in a laboratory mouse model. However, after two weeks, researchers also observed that C60 <\/sub>actually began to augment tumor growth after intraperitoneal administration in an in vitro<\/em> study. This demonstrates a significant difference between C60<\/sub>\u2019s anticancer activity on an intracellular level and its capacity to potentiate tumor growth.<\/p>\n\n\n\nTo sum, C60<\/sub>\u2019s anti-cancer and chemoprotective capabilities appear to be complex and situation-dependent. For this reason, much more research is necessary before C60<\/sub> fullerene can be considered a safe and effective adjunct cancer treatment.<\/p>\n\n\n\nMultiple sclerosis<\/strong><\/h3>\n\n\n\nMultiple sclerosis is a disease affecting the central nervous system, in which the immune system mistakenly attacks the protective myelin sheath that covers nerve fibers. This results in nerve scarring that inhibits effective signaling between the brain and body. Symptoms may include vision loss, chronic pain, impaired coordination, and chronic fatigue. While there is no cure for multiple sclerosis (MS) to date, research has indicated a strong need for effective antioxidant therapies in combating the oxidative stress associated with the disease. Some researchers have theorized that C60<\/sub> may be an effective treatment for MS based on its antioxidant potency. Multiple sclerosis is also associated with free radical toxicity, a quality that may be ameliorated by C60<\/sub>\u2019s mechanism of action. However, to date there have been no clinical trials proving or disproving buckminsterfullerene\u2019s efficacy in treating multiple sclerosis.<\/p>\n\n\n\nReminder<\/h4>Make sure to check for C60’s side effects and toxicity before intake. Consult your doctor.<\/p><\/div><\/div>\n\n\n\n
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Anti-Inflammatory<\/strong><\/h3>\n\n\n\nIn addition to being a powerful antioxidant, C60<\/sub> fullerene has demonstrated anti-inflammatory activities. A 2016 study published by the Journal of Nanobiotechnology <\/em>observed the therapeutic effects of a water-soluble form of fullerene C60<\/sub> in ROS (reactive oxygen species)-dependent inflammation. A C60<\/sub> solution was administered subcutaneously and epicutaneously to mice suffering from atopic dermatitis. Results indicated that C60<\/sub> was able to restore functionality of the skin barrier, shifting immune response from Th2 cytokine production to increased Th1 cytokine production.<\/p>\n\n\n\nA study published by Basic & Clinical Pharmacology & Toxicology<\/em> yielded similar results. Researchers examined the therapeutic effects of a saline solution of C60<\/sub> in mice with neutrophilic lung inflammation induced by quartz. Results indicated that, via free radical scavenging, C60<\/sub> was able to attenuate neutrophilic lung inflammation and neutralize the pro-inflammatory actions of quartz-induced ROS.<\/p>\n\n\n\nSkin<\/strong><\/h3>\n\n\n\nA 2010 study published by the Journal of Nanoscience and Nanotechnology <\/em>evaluated a highly purified and organic-solvent-free treatment of C60<\/sub> fullerene and its capabilities as a cosmetic antioxidant. The study was conducted with twenty-three Japanese women in their thirties; they were enrolled in an eight-week trial assessing the anti-wrinkle efficacy of LF-SQ (squalane-dissolved C60<\/sub> fullerene) in which the blended cream was applied twice daily on one side of the face. Researchers evaluated the following parameters: trans-epidermal water loss (TEWL), moisture levels of the stratum corneum, and visco-elasticity of the cheeks. Conclusions yielded that LF-SQ was not effective by the fourth week, but began to produce results by the 8th<\/sup> week. Specifically, the LF-SQ preparation reduced skin roughness by a statistically significant margin (p<0.05).<\/p>\n\n\n\nC60<\/sub> fullerene is often referred to as a \u201cradical sponge\u201d in terms of its relationship with the skin. While C60<\/sub> fullerene has been the subject of intensive research in the cosmetic industry over the past few years, some researchers have also demonstrated interest in its free radical scavenging activity in relationship to melanogenesis in human skin cells. A 2006 study published by Fullerenes, Nanotubes and Carbon Nanostructures <\/em>evaluated the inhibitory effect of water-soluble C60<\/sub> on UV-induced apopotosis-like cell death against HaCaT cells. Researchers observed that the water-soluble fullerene treatment inhibited cell death and attenuated melanogenesis in human skin cells without any observable cytotoxicity. This information indicates that C60<\/sub> fullerene may have anti-melanoma activities as well as skin-lightening properties. However, a safety evaluation of C60<\/sub> fullerene and its impacts on the human dermis has yet to be conducted.<\/p>\n\n\n\nDiabetes<\/strong><\/h3>\n\n\n\nSome researchers have proposed the use of C60<\/sub> in the treatment of diabetes mellitus and its complications based on the allotrope\u2019s action on oxidative stress. In a 2012 study conducted by the Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU) in Kiev, researchers assessed the antioxidant effects of hydrated C60<\/sub> fullerene (C60<\/sub>HyFn) in the brains and livers of male Wistar rats with streptozotocin-induced hyperglycemia (streptozotocin also induced oxidative damage in the lipids and proteins of the rats\u2019 central nervous system and liver). Researchers found that therapeutically-administered C60<\/sub> fullerene restored gilial fibrillary acidic proteins to their normal levels by lowering astrogliosis (a destructive increase of astrocytes due to trauma or infection) caused by hyperglycemia. However, they also observed that C60<\/sub> did not remediate blood glucose levels in diabetic rats and therefore did not attenuate the primary causal factor of metabolic disturbance.<\/p>\n\n\n\nAnother study published by Toxicology<\/em> explored diabetes mellitus as it relates to male sexual dysfunction and the ability of hydrated C60<\/sub> fullerene in eliminating hyperglycemia-induced testicular dysfunction in male Wistar rats. Rats received an aqueous treatment of C60<\/sub> at a dose of 4\u03bcg\/kg daily for five weeks after hyperglycemia was induced with streptozotocin. Researchers found that levels of serum testosterone, testicular-reduced glutathione, and alpha-tocopherol were significantly reduced under STZ-induced diabetes, but that C60<\/sub> had a significant corrective effect on these parameters.<\/p>\n\n\n\nThough results of these and other studies are not definitively conclusive, there is some clinical evidence to indicate that C60<\/sub> may play a measured role in reducing diabetes-induced oxidative stress and complications of diabetes mellitus. However, it is unlikely that C60<\/sub> fullerene treatment is able to ameliorate the cause of hyperglycemic diabetes.<\/p>\n\n\n\nWeight Loss<\/strong><\/h3>\n\n\n\nBuckminsterfullerene oil\u2019s protective action against reactive oxygen species (ROS) has led many researchers to hypothesize that it may yield a weight management effect, based on the assumption that reactive oxygen species play a key factor in the development of obesity. A study published by Molecular and Cellular Biochemistry<\/em> examined intracellular lipid accumulation in a laboratory mouse model and observed buckminsterfullerene\u2019s ability to suppress this process. Researchers found that polyhydroxylated fullerene C60<\/sub> suppressed lipid accumulation and subsequent PPAR\u03b32 expression, ultimately decreasing the spontaneous differentiation of preadipocytes into adipocytes (or fat storage cells).<\/p>\n\n\n\n <\/strong>A 2010 study published by Biomaterials<\/em> yielded similar results. Researchers explored the effects of squalane-dissolved fullerene C60<\/sub> on adipose conversion in inflammatory adipose-tissue and stromal preadipocyte-U937 lymphoma cell cultures. They found that squalane-dissolved fullerene C60<\/sub> inhibited macrophage activation and adipogenesis in the OP9-U937 culture. Researchers concluded that squalane-C60<\/sub> fullerene may have potential as a therapeutic option in the treatment of metabolic syndrome and\/or obesity-related diseases.<\/p>\n\n\n\nMacular degeneration<\/strong><\/h3>\n\n\n\nMacular degeneration is a condition caused by the slow degradation of the eye\u2019s central retina, and is the leading cause of vision loss and blindness in North America. In recent years, individuals studying the degeneration of the human eye have turned to nanotechnology as a possible regenerative therapy.<\/p>\n\n\n\n
To date, no studies have pointed to C60<\/sub> specifically as a therapeutic agent in the treatment of macular degeneration. However, a 2005 study published by Chemistry & Biology<\/em> did observe the antimicrobial and photosensitive activity of C60<\/sub> compounds against gram-positive bacteria, gram-negative bacteria, and fungi. Results pointed C60<\/sub> fullerene as an effective and selective antimicrobial photosensitizer, meaning it may have a similar mechanism to medications that use photodynamic therapy to ameliorate macular degeneration (such as verteporfin, a benzoporphyrin derivative). However, more clinical evidence is needed before C60<\/sub> fullerene can be considered a safe and efficacious photodynamic therapy in the treatment of retinal degeneration.<\/p>\n\n\n\nDepression<\/strong><\/h3>\n\n\n\nThere is no clinical evidence to suggest that buckminsterfullerene or its derivatives have an antidepressant effect on patients. A 2008 study published by the Journal of Nanoscience and Nanotechnology<\/em> found that, while intracerebral injection of C60<\/sub> in laboratory rats did increase dopamine turnover rates in the hypothalamus and serotonin turnover rates in the hypothalamus, cerebral cortex, and hippocampus, intraperitoneal injection decreased dopamine turnover rates in the hippocampus. These data suggest that C60<\/sub> fullerene cannot cross the blood-brain barrier and can only act on the central nervous system when administered intracerebrally.<\/p>\n\n\n\nMore data is required to elucidate buckminsterfullerene\u2019s exact mechanism of action in the central nervous systems of mammals.<\/p>\n\n\n\n
C60 Dosage<\/strong><\/h2>\n\n\n\nMost individuals opt to take 1 to 3 ml per day of buckminsterfullerene olive oil. The proper dosage of C60<\/sub> oil has not been clinically validated or scientifically evaluated, so this is based strictly on anecdotal information from individuals taking C60 <\/sub>olive oil.<\/p>\n\n\n\nC60<\/sub> oil should not be situated near any heat-producing appliances. Further, refrigeration will cause the substance to congeal.<\/p>\n\n\n\nC60 Side Effects, Toxicity, Safety and Warning<\/strong><\/h2>\n\n\n\nWhile C60<\/sub> has exhibited numerous beneficial effects in the laboratory and is easily absorbed into mammalian cells, it is important to remember that its permeability represents potential dangers to the cell membrane if administered incorrectly or excessively. Further, buckminsterfullerene is, in essence, a carbon allotrope, and has some similar structural properties to asbestos fibers. When dissolved in oil, C60<\/sub> fullerene is believed to be relatively safe as oil affinity prevents the molecular clustering that leads to crystallization and toxicity. However, research into the toxicity of C60 <\/sub>oil is relatively limited, and it is recommended that interested patients consult thoroughly with a physician and be highly conscious of factors such as manufacturers and product purity. Though C60 <\/sub>fullerene has yielded substantial benefits in laboratory mouse models, it is important to note that these results arise under meticulously controlled conditions. Further, there have been some anecdotal reports of tumorigenesis resulting from C60<\/sub> oil consumption.<\/p>\n\n\n\nThough C60<\/sub> is believed to be safest in its oil form, it is vital to remember that oxidized oil is not invulnerable to toxicity. Polyphenols in oils decline very quickly in a matter of months, and this process is only accelerated by the introduction of light and oxygen. Many researchers are currently evaluating alternative vehicles for detoxifying C60<\/sub> and delivering its beneficial bioactive assets to the human body. Further, there is some concern among global administrative bodies regarding the integration of C60<\/sub> fullerene into ingestible food sources.<\/p>\n\n\n\nThe Norwegian Scientific Committee for Food Safety, for example, determined after an analysis of existing toxicological and microbiological data that most key safety information is lacking in relationship to this supplement. The European Food Safety Authority concluded that the application of C